A History of Science, vol 4 - Henry Smith Williams (epub e ink reader .txt) 📗
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in no sense causal. This opinion of the great German chemist was
in a measure substantiated by experiments of his compatriot
Helmholtz, whose earlier experiments confirmed, but later ones
contradicted, the observations of Schwann, and this combined
authority gave the vitalistic conception a blow from which it had
not rallied at the time when Pasteur entered the field. Indeed,
it was currently regarded as settled that the early students of
the subject had vastly over-estimated the importance of
micro-organisms.
And so it came as a new revelation to the generality of
scientists of the time, when, in 1857 and the succeeding
half-decade, Pasteur published the results of his researches, in
which the question had been put to a series of altogether new
tests, and brought to unequivocal demonstration.
He proved that the micro-organisms do all that his most
imaginative predecessors had suspected, and more. Without them,
he proved, there would be no fermentation, no putrefaction—no
decay of any tissues, except by the slow process of oxidation. It
is the microscopic yeast-plant which, by seizing on certain atoms
of the molecule, liberates the remaining atoms in the form of
carbonic-acid and alcohol, thus effecting fermentation; it is
another microscopic plant—a bacterium, as Devaine had christened
it—which in a similar way effects the destruction of organic
molecules, producing the condition which we call putrefaction.
Pasteur showed, to the amazement of biologists, that there are
certain forms of these bacteria which secure the oxygen which all
organic life requires, not from the air, but by breaking up
unstable molecules in which oxygen is combined; that
putrefaction, in short, has its foundation in the activities of
these so-called anaerobic bacteria.
In a word, Pasteur showed that all the many familiar processes of
the decay of organic tissues are, in effect, forms of
fermentation, and would not take place at all except for the
presence of the living micro-organisms. A piece of meat, for
example, suspended in an atmosphere free from germs, will dry up
gradually, without the slightest sign of putrefaction, regardless
of the temperature or other conditions to which it may have been
subjected. Let us witness one or two series of these experiments
as presented by Pasteur himself in one of his numerous papers
before the Academy of Sciences.
EXPERIMENTS WITH GRAPE SUGAR“In the course of the discussion which took place before the
Academy upon the subject of the generation of ferments properly
so-called, there was a good deal said about that of wine, the
oldest fermentation known. On this account I decided to disprove
the theory of M. Fremy by a decisive experiment bearing solely
upon the juice of grapes.
“I prepared forty flasks of a capacity of from two hundred and
fifty to three hundred cubic centimetres and filled them half
full with filtered grape-must, perfectly clear, and which, as is
the case of all acidulated liquids that have been boiled for a
few seconds, remains uncontaminated although the curved neck of
the flask containing them remain constantly open during several
months or years.
“In a small quantity of water I washed a part of a bunch of
grapes, the grapes and the stalks together, and the stalks
separately. This washing was easily done by means of a small
badger’s-hair brush. The washing-water collected the dust upon
the surface of the grapes and the stalks, and it was easily shown
under the microscope that this water held in suspension a
multitude of minute organisms closely resembling either fungoid
spores, or those of alcoholic Yeast, or those of Mycoderma vini,
etc. This being done, ten of the forty flasks were preserved for
reference; in ten of the remainder, through the straight tube
attached to each, some drops of the washing-water were
introduced; in a third series of ten flasks a few drops of the
same liquid were placed after it had been boiled; and, finally,
in the ten remaining flasks were placed some drops of grape-juice
taken from the inside of a perfect fruit. In order to carry out
this experiment, the straight tube of each flask was drawn out
into a fine and firm point in the lamp, and then curved. This
fine and closed point was filed round near the end and inserted
into the grape while resting upon some hard substance. When the
point was felt to touch the support of the grape it was by a
slight pressure broken off at the point file mark. Then, if care
had been taken to create a slight vacuum in the flask, a drop of
the juice of the grape got into it, the filed point was
withdrawn, and the aperture immediately closed in the alcohol
lamp. This decreased pressure of the atmosphere in the flask was
obtained by the following means: After warming the sides of the
flask either in the hands or in the lamp-flame, thus causing a
small quantity of air to be driven out of the end of the curved
neck, this end was closed in the lamp. After the flask was
cooled, there was a tendency to suck in the drop of grape-juice
in the manner just described.
“The drop of grape-juice which enters into the flask by this
suction ordinarily remains in the curved part of the tube, so
that to mix it with the must it was necessary to incline the
flask so as to bring the must into contact with the juice and
then replace the flask in its normal position. The four series of
comparative experiments produced the following results:
“The first ten flasks containing the grape-must boiled in pure
air did not show the production of any organism. The grape-must
could possibly remain in them for an indefinite number of years.
Those in the second series, containing the water in which the
grapes had been washed separately and together, showed without
exception an alcoholic fermentation which in several cases began
to appear at the end of forty-eight hours when the experiment
took place at ordinary summer temperature. At the same time that
the yeast appeared, in the form of white traces, which little by
little united themselves in the form of a deposit on the sides of
all the flasks, there were seen to form little flakes of
Mycellium, often as a single fungoid growth or in combination,
these fungoid growths being quite independent of the must or of
any alcoholic yeast. Often, also, the Mycoderma vini appeared
after some days upon the surface of the liquid. The Vibria and
the lactic ferments properly so called did not appear on account
of the nature of the liquid.
“The third series of flasks, the washing-water in which had been
previously boiled, remained unchanged, as in the first series.
Those of the fourth series, in which was the juice of the
interior of the grapes, remained equally free from change,
although I was not always able, on account of the delicacy of the
experiment, to eliminate every chance of error. These experiments
cannot leave the least doubt in the mind as to the following
facts:
Grape-must, after heating, never ferments on contact with the
air, when the air has been deprived of the germs which it
ordinarily holds in a state of suspension.
“The boiled grape-must ferments when there is introduced into it
a very small quantity of water in which the surface of the grapes
or their stalks have been washed.
“The grape-must does not ferment when this washing-water has been
boiled and afterwards cooled.
“The grape-must does not ferment when there is added to it a
small quantity of the juice of the inside of the grape.
“The yeast, therefore, which causes the fermentation of the
grapes in the vintage-tub comes from the outside and not from the
inside of the grapes. Thus is destroyed the hypothesis of MM.
Trecol and Fremy, who surmised that the albuminous matter
transformed itself into yeast on account of the vital germs which
were natural to it. With greater reason, therefore, there is no
longer any question of the theory of Liebig of the transformation
of albuminoid matter into ferments on account of the oxidation.”
FOREIGN ORGANISMS AND THE WORT OF BEER
“The method which I have just followed,” Pasteur continues, “in
order to show that there exists a correlation between the
diseases of beer and certain microscopic organisms leaves no room
for doubt, it seems to me, in regard to the principles I am
expounding.
“Every time that the microscope reveals in the leaven, and
especially in the active yeast, the production of organisms
foreign to the alcoholic yeast properly so called, the flavor of
the beer leaves something to be desired, much or little,
according to the abundance and the character of these little
germs. Moreover, when a finished beer of good quality loses after
a time its agreeable flavor and becomes sour, it can be easily
shown that the alcoholic yeast deposited in the bottles or the
casks, although originally pure, at least in appearance, is found
to be contaminated gradually with these filiform or other
ferments. All this can be deduced from the facts already given,
but some critics may perhaps declare that these foreign ferments
are the consequences of the diseased condition, itself produced
by unknown causes.
“Although this gratuitous hypothesis may be difficult to uphold,
I will endeavor to corroborate the preceding observations by a
clearer method of investigation. This consists in showing that
the beer never has any unpleasant taste in all cases when the
alcoholic ferment properly so called is not mixed with foreign
ferments; that it is the same in the case of wort, and that wort,
liable to changes as it is, can be preserved unaltered if it is
kept from those microscopic parasites which find in it a suitable
nourishment and a field for growth.
“The employment of this second method has, moreover, the
advantage of proving with certainty the proposition that I
advanced at first—namely, that the germs of these organisms are
derived from the dust of the atmosphere, carried about and
deposited upon all objects, or scattered over the utensils and
the materials used in a brewery-materials naturally charged with
microscopic germs, and which the various operations in the
store-rooms and the malt-house may multiply indefinitely.
“Let us take a glass flask with a long neck of from two hundred
and fifty to three hundred cubic centimetres capacity, and place
in it some wort, with or without hops, and then in the flame of a
lamp draw out the neck of the flask to a fine point, afterwards
heating the liquid until the steam comes out of the end of the
neck. It can then be allowed to cool without any other
precautions; but for additional safety there can be introduced
into the little point a small wad of asbestos at the moment that
the flame is withdrawn from beneath the flask. Before thus
placing the asbestos it also can be passed through the flame, as
well as after it has been put into the end of the tube. The air
which then first re-enters the flask will thus come into contact
with the heated glass and the heated liquid, so as to destroy the
vitality of any dust germs that may exist in the air. The air
itself will re-enter very gradually, and slowly enough to enable
any dust to be taken up by the drop of water which the air forces
up the curvature of the tube. Ultimately the tube will be dry,
but the re-entering of the air will be so slow that the particles
of dust will fall upon the sides of the tube. The experiments
show that with this kind of vessel, allowing free communication
with the air, and the dust not being allowed to enter, the dust
will not enter at all events for a period of ten or twelve
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